7 Tips to Make Crazy Money in Photography - download pdf or read online

By Jonathan Souza

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In particular, different strains of mice respond differentially to drug treatment, and acut7e sleep deprivation alters the response to a number of drugs of abuse. However, essentially nothing is known about how the genetic background affects the response to drugs of abuse in animals with disrupted sleep. Examining the effects of sleep disruption on the response to drugs of abuse in mice with different genetic backgrounds is particularly important since one of the hallmarks of substance abuse in humans is disrupted sleep.

Generate_Screen • Project Title: IMPORTANCE OF SLEEP & GENOTYPE IN DRUG ABUSE STUDIES Principal Investigator & Institution: Dugovic, Christine; None; Northwestern University Office of Sponsored Programs Chicago, Il 60611 Timing: Fiscal Year 2001; Project Start 30-SEP-2001; Project End 31-AUG-2004 Summary: (provided by applicant): It has been well established that both genetic background and the environment can have pronounced effects on how mice respond to drugs of abuse, including both psychostimulants and the opiates.

In vivo infusion of an antisense to c-fos attenuates IL- 1-induced FOS activation in the PVN. Taken together, these data indicate that morphine use attenuates both the action and expression of IL- 1 in the brain. It has been suggested that the HPA is involved in morphine's effect on immune responses. Leukocyte-endothelial adhesion (LEA) is the initial step of the immune response cascade. Our continuation studies will address whether blockage of IL-1-induced FOS activation in the PVN has biological significance related to the HPA axis and LEA in the course of morphine tolerance with the following specific aims: (1) to characterize the AP-1 binding activity in the rat brain following treatment with either morphine or lL-1 using gel-shift mobility assay and Western blot analysis; (2) to characterize the effects of blockage of FOS activation on IL-1's modulation of the HPA axis by examining the mRNA levels of corticotropin releasing factor (CRF), proopiomelanocortin (POMC) by reverse-transcriptase polymerase chain reaction (RTPCR), CRF peptide, adrenocorticotropic hormone (ACTH), and corticosterone by radioimmunoassay (RIA); (3) to characterize the modulatory role of the HPA products, such as glucocorticoid, on LEA both in animal and in vitro cell models using intravital microscopy; cell culture and light microscopy; and (4) to characterize the expression of 32 Morphine IL- 1 in the central nervous system and periphery following chronic exposure to morphine using immunocytochemical staining, RT-PCR and Enzyme-linked immunosorbant assay (ELISA) techniques.

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7 Tips to Make Crazy Money in Photography by Jonathan Souza


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