Alton Meister's Advances in Enzymology and Related Areas of Molecular PDF

By Alton Meister

ISBN-10: 0470123028

ISBN-13: 9780470123027

ISBN-10: 047189012X

ISBN-13: 9780471890126

Maintains the authoritative sequence that studies learn into enzymology and comparable components of molecular biology. offers six papers via top gurus.


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Additional info for Advances in Enzymology and Related Areas of Molecular Biology, Volume 56

Sample text

C). Like other PLP-enzymes, Ala(CN) synthase is sensitive to carbonyl-modifying reagents, particularly those structurally analogous to primary substrates. Although moderately susceptible to inhibition Figure 9. Effect of p-mercuribenzoate (p-CMB) upon production of H2S (curve 1) and CNS- (curve 2) by Ala(CN) synthase (60 mu) from L-cystine (20 mM) and cyanide (50 mM). ) Ordinates: AHtS and ACNS- in pmol from 0-20 min. 26 a From reference 28. 2 ml aminoacylase I solution. Incubation: 1 hat 30°C.

See refs. ) We used the purified enzyme in comparative studies regarding interactions of PLP-dependent lyases with diverse ligands, isotopic hydrogen exchange, and so forth. The results are presented in relevant parts of Section 111. In 1979-1982, Dwivedi and coworkers (91a) selected, by means of DNA hybridization and cloning techniques, an E. coli strain which is able to overproduce P-cystathionaseby 100-fold. min-'/mg (100-fold higher than their best preparations of y-cystathionase from rat liver); but cf.

12), is recovered as a volatile product responsible for the pungent smell and antibacterial properties of garlic. Alliinase was shown by Goryachenkova (83) to be a PLP-dependent enzyme. In onion, garlic, and tissues of related species of the genus Allium, homologous Salkylcysteine sulfoxides (methyl, ethyl, propyl, butyl analogs, etc. of alliin) are cleaved by similar lyases (with varying substrate specificities) to yield characteristically smelling allicin-like antibiotics (bis-alkyl-disulfoxides) (85).

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Advances in Enzymology and Related Areas of Molecular Biology, Volume 56 by Alton Meister

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