Download PDF by Erin K. Marasco, Claudia Schmidt-Dannert (auth.), R.: Applications of Plant Metabolic Engineering

By Erin K. Marasco, Claudia Schmidt-Dannert (auth.), R. Verpoorte, A.W. Alfermann, T.S. Johnson (eds.)

ISBN-10: 1402060300

ISBN-13: 9781402060304

ISBN-10: 1402060319

ISBN-13: 9781402060311

Written by means of major foreign specialists within the box of plant metabolic engineering, this ebook discusses, typically, functions of plant metabolic engineering. Metabolic engineering, that's a part of moment iteration biotechnology, has made numerous contributions at simple point in addition to at conceptualising items ever considering its emergence within the early Nineteen Eighties. purposes caused by metabolic engineering are anticipated to play a vital function in destiny for plant breeding, e.g. for enhanced resistance or more desirable qualities bearing on overall healthiness selling ingredients, and creation of good chemical compounds reminiscent of medications, flavors and fragrances.

Considering the great development that has been made when you consider that our first ebook within the yr 2000 urgency used to be felt to compile innovative study in plant metabolic engineering in utilized points as a convenient reference ebook. This e-book has been predominantly devoted to utilized examine of metabolic engineering, and part of it's been dispensed for primary learn that has strength functions. This ebook is a distinct resource of data for all these concerned with vegetation as chemical factories of excellent chemical compounds and likewise to more matured scientists who paintings on transgenic crops, in addition to, senior researchers, graduate scholars, post-doctroral scholars, academics and scholars to boot.

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The isoprenoid precursor geranylgeranyl diphosphate (GGDP) is cyclized to taxa-4(5), 11(12)-diene in the first committed step in the biosynthesis of Taxol. This reaction is catalyzed by taxadiene synthase, an enzyme first cloned and characterized a decade ago (Wildung and Croteau, 1996). The taxane skeleton is then further functionalized by a series of eight oxidative modifications catalyzed by cytochrome P450 enzymes, three CoA dependent acylations and side chain additions catalyzed by CoA-thioester dependent transferases (Walker and Croteau, 2001) (Figure 8).

Back K, Chappell J (1995) Cloning and bacterial expression of a sesquiterpene cyclase from Hyoscyamus muticus and its molecular comparison to related terpene cyclases. J Biol Chem 270(13): 7375–7381. Bayer A, Ma XY, Stöckigt J (2004) Acetyltransfer in natural product biosynthesis – functional cloning and molecular analysis of vinorine synthase. Bioorg Med Chem 12(10):2787–2795. Becker JVW, Armstrong GO, Van Der Merwe MJ, et al. (2003) Metabolic engineering of Saccharomyces cerevisiae for the synthesis of the wine-related antioxidant resveratrol.

Flavonoid pathway elucidation originally focused on anthocyanins, the brightly colored flavonoid structures that attract pollinators and seed dispersers to plants. Identification of genes and regulatory mechanisms involved in anthocyanin biosynthesis was greatly aided by the visible, screenable colored phenotypes (Forkmann and Martens, 2001). In recent years, many flavonoid genes have been have been cloned from diverse plant species and heterologously expressed in microbial hosts for enzymatic characterization.

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Applications of Plant Metabolic Engineering by Erin K. Marasco, Claudia Schmidt-Dannert (auth.), R. Verpoorte, A.W. Alfermann, T.S. Johnson (eds.)


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